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Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota.

ABSTRACT: Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus extract, and 62 metabolites by human intestinal microbiota were detected. Two polyynes, falcarindiol and oplopandiol were recognized as the main components of O. elatus, whose metabolites are further illustrated. Several metabolic pathways were proposed to generate the detected metabolites, including methylation, hydrogenation, demethylation, dehydroxylation, and hydroxylation. These findings indicated that intestinal microbiota might play an essential role in mediating the bioactivity of O. elatus.

SUBMITTER: Wan JY

PROVIDER: S-EPMC8627129 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota.

Wan Jin-Yi JY Wan Jing-Xuan JX Wang Shilei S Wang Xiaolu X Guo Wenqian W Ma Han H Wu Yuqi Y Wang Chong-Zhi CZ Qi Lian-Wen LW Li Ping P Yao Haiqiang H Yuan Chun-Su CS

PeerJ 20211124

Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus ...[more]

PMID: 34900430

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Dataset Information

Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota.

Publications

Chemical profiling of root bark extract from <i>Oplopanax elatus</i> and its <i>in vitro</i> biotransformation by human intestinal microbiota.

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