Re-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters.

ABSTRACT: Streptomyces species have attracted considerable interest as a reservoir of medically important secondary metabolites, which are even diverse and different between strains. Here, we reassess ten Streptomyces venezuelae strains by presenting the highly resolved classification, using 16S rRNA sequencing, MALDI-TOF MS protein profiling, and whole-genome sequencing. The results revealed that seven of the ten strains were misclassified as S. venezuelae species. Secondary metabolite biosynthetic gene cluster (smBGC) mining and targeted LC-MS/MS based metabolite screening of S. venezuelae and misclassified strains identified in total 59 secondary metabolites production. In addition, a comparison of pyrrolamide-type antibiotic BGCs of four misclassified strains,

SUBMITTER: Lee N 

PROVIDER: S-EPMC8627960 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature