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Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin.

ABSTRACT: As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-genome sequencing revealed an average nucleotide identity (ANI) value of 89% with A. macra, the highest among related species. Further phenotypic and chemotaxonomic analyses confirmed that the isolate represents a previously uncharacterised species in the genus Actinomadura, for which the name Actinomadura graeca sp. nov. is proposed (type strain 32-07T). The G+C content of A. graeca 32-07 is 72.36%. The cell wall contains DL-diaminopimelic acid, intracellular sugars are glucose, ribose and galactose, the predominant menaquinone is MK-9(H6), the major cellular lipid is phosphatidylinositol and fatty acids consist mainly of hexadecanoic acid. No mycolic acid was detected. Furthermore, A. graeca 32-07 has been confirmed as a novel producer of the non-ribosomal peptide antibiotic zelkovamycin and we report herein a provisional description of the unique biosynthetic gene cluster.

SUBMITTER: Tarantini FS

PROVIDER: S-EPMC8631618 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin.

Tarantini Francesco Saverio FS Brunati Mara M Taravella Anna A Carrano Lucia L Parenti Francesco F Hong Kar Wai KW Williams Paul P Chan Kok Gan KG Heeb Stephan S Chan Weng C WC

PloS one 20211130 11

As part of a screening programme for antibiotic-producing bacteria, a novel Actinomadura species was discovered from a soil sample collected in Santorini, Greece. Preliminary 16S rRNA gene sequence comparisons highlighted Actinomadura macra as the most similar characterised species. However, whole-genome sequencing revealed an average nucleotide identity (ANI) value of 89% with A. macra, the highest among related species. Further phenotypic and chemotaxonomic analyses confirmed that the isolate ...[more]

PMID: 34847153

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Dataset Information

Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin.

Publications

Actinomadura graeca sp. nov.: A novel producer of the macrocyclic antibiotic zelkovamycin.

Similar Datasets

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