Transcriptome-wide In Vitro Effects of Aspirin on Patient-derived Normal Colon Organoids.

ABSTRACT: Mechanisms underlying aspirin chemoprevention of colorectal cancer remain unclear. Prior studies have been limited because of the inability of preclinical models to recapitulate human normal colon epithelium or cellular heterogeneity present in mucosal biopsies. To overcome some of these obstacles, we performed in vitro aspirin treatment of colon organoids derived from normal mucosal biopsies to reveal transcriptional networks relevant to aspirin chemoprevention. Colon organoids derived from 38 healthy individuals undergoing endoscopy were treated with 50 μmol/L aspirin or vehicle control for 72 hours and subjected to bulk RNA sequencing. Paired regression analysis using DESeq2 identified differentially expressed genes (DEG) associated with aspirin treatment. Cellular composition wa

SUBMITTER: Devall MAM 

PROVIDER: S-EPMC8639779 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature