Transcriptional and chromatin-based partitioning mechanisms uncouple protein scaling from cell size.

ABSTRACT: Biosynthesis scales with cell size such that protein concentrations generally remain constant as cells grow. As an exception, synthesis of the cell-cycle inhibitor Whi5 "sub-scales" with cell size so that its concentration is lower in larger cells to promote cell-cycle entry. Here, we find that transcriptional control uncouples Whi5 synthesis from cell size, and we identify histones as the major class of sub-scaling transcripts besides WHI5 by screening for similar genes. Histone synthesis is thereby matched to genome content rather than cell size. Such sub-scaling proteins are challenged by asymmetric cell division because proteins are typically partitioned in proportion to newborn cell volume. To avoid this fate, Whi5 uses chromatin-binding to partition similar protein amounts to each ne

SUBMITTER: Swaffer MP 

PROVIDER: S-EPMC8642314 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature