Dataset Information

In vitro transmission of 16SrIV phytoplasmas to coconut plants by Haplaxius crudus in Yucatan, Mexico.

ABSTRACT: The coconut palm is an important crop worldwide. In America, it is affected by lethal yellowing (LY) disease, associated with the presence of 16SrIV ribosomal group phytoplasmas. Studies in Florida using insect-proof cages indicate Haplaxius crudus as a vector of LY phytoplasmas to palm species, including coconut. Here, an in vitro transmission system was used to verify that H. crudus collected in Yucatan, Mexico, transmit 16SrIV phytoplasmas to coconut. Three transmission trials were carried out using micropropagated coconut plants. In each trial, at least one plant was positive to 16SrIV phytoplasmas. In total, there were 4 positive plants out of 34 exposed to insects, and the phytoplasma presence was detected in root, stem, and leaf tissues. The phytoplasmas identified were 16SrIV-A and 16SrIV-D, both found in both plants and insects. In each assay where a plant was positive for either 16SrIV-A or 16SrIV-D, the same phytoplasma was present in the insect or insects used in this assay. This is the first demonstration of transmission of LY phytoplasmas to coconut plants by H. crudus in Mexico and with an in vitro system.

Supplementary information

The online version contains supplementary material available at 10.1007/s13205-021-03069-z.

SUBMITTER: Narvaez M

PROVIDER: S-EPMC8643390 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

In vitro transmission of 16SrIV phytoplasmas to coconut plants by Haplaxius crudus in Yucatan, Mexico.

Narváez María M Nic-Matos Germán G Oropeza Carlos C

3 Biotech 20211205 1

The coconut palm is an important crop worldwide. In America, it is affected by lethal yellowing (LY) disease, associated with the presence of 16SrIV ribosomal group phytoplasmas. Studies in Florida using insect-proof cages indicate Haplaxius crudus as a vector of LY phytoplasmas to palm species, including coconut. Here, an in vitro transmission system was used to verify that H. crudus collected in Yucatan, Mexico, transmit 16SrIV phytoplasmas to coconut. Three transmission trials w ...[more]

PMID: 34926118

Similar Datasets

Project description:Dengue vaccines will soon provide a new tool for reducing dengue disease, but the effectiveness of widespread vaccination campaigns has not yet been determined. We developed an agent-based dengue model representing movement of and transmission dynamics among people and mosquitoes in Yucatán, Mexico, and simulated various vaccine scenarios to evaluate effectiveness under those conditions. This model includes detailed spatial representation of the Yucatán population, including the location and movement of 1.8 million people between 375,000 households and 100,000 workplaces and schools. Where possible, we designed the model to use data sources with international coverage, to simplify re-parameterization for other regions. The simulation and analysis integrate 35 years of mild and severe case data (including dengue serotype when available), results of a seroprevalence survey, satellite imagery, and climatological, census, and economic data. To fit model parameters that are not directly informed by available data, such as disease reporting rates and dengue transmission parameters, we developed a parameter estimation toolkit called AbcSmc, which we have made publicly available. After fitting the simulation model to dengue case data, we forecasted transmission and assessed the relative effectiveness of several vaccination strategies over a 20 year period. Vaccine efficacy is based on phase III trial results for the Sanofi-Pasteur vaccine, Dengvaxia. We consider routine vaccination of 2, 9, or 16 year-olds, with and without a one-time catch-up campaign to age 30. Because the durability of Dengvaxia is not yet established, we consider hypothetical vaccines that confer either durable or waning immunity, and we evaluate the use of booster doses to counter waning. We find that plausible vaccination scenarios with a durable vaccine reduce annual dengue incidence by as much as 80% within five years. However, if vaccine efficacy wanes after administration, we find that there can be years with larger epidemics than would occur without any vaccination, and that vaccine booster doses are necessary to prevent this outcome.

Dataset Information

In vitro transmission of 16SrIV phytoplasmas to coconut plants by Haplaxius crudus in Yucatan, Mexico.

Supplementary information

Publications

In vitro transmission of 16SrIV phytoplasmas to coconut plants by <i>Haplaxius crudus</i> in Yucatan, Mexico.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets