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High throughput and quantitative enzymology in the genomic era.

ABSTRACT: Accurate predictions from models based on physical principles are the ultimate metric of our biophysical understanding. Although there has been stunning progress toward structure prediction, quantitative prediction of enzyme function has remained challenging. Realizing this goal will require large numbers of quantitative measurements of rate and binding constants and the use of these ground-truth data sets to guide the development and testing of these quantitative models. Ground truth data more closely linked to the underlying physical forces are also desired. Here, we describe technological advances that enable both types of ground truth measurements. These advances allow classic models to be tested, provide novel mechanistic insights, and place us on the path toward a predictive understanding of enzyme structure and function.

SUBMITTER: Mokhtari DA

PROVIDER: S-EPMC8648990 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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High throughput and quantitative enzymology in the genomic era.

Mokhtari D A DA Appel M J MJ Fordyce P M PM Herschlag D D

Current opinion in structural biology 20210927

Accurate predictions from models based on physical principles are the ultimate metric of our biophysical understanding. Although there has been stunning progress toward structure prediction, quantitative prediction of enzyme function has remained challenging. Realizing this goal will require large numbers of quantitative measurements of rate and binding constants and the use of these ground-truth data sets to guide the development and testing of these quantitative models. Ground truth data more ...[more]

PMID: 34592682

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Dataset Information

High throughput and quantitative enzymology in the genomic era.

Publications

High throughput and quantitative enzymology in the genomic era.

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