In-vitro propagation and phytochemical profiling of a highly medicinal and endemic plant species of the Himalayan region (Saussurea costus).

ABSTRACT: Efficient protocols for callus induction and micro propagation of Saussurea costus (Falc.) Lipsch were developed and phytochemical diversity of wild and in-vitro propagated material was investigated. Brown and red compact callus was formed with frequency of 80-95%, 78-90%, 70-95% and 65-80% from seeds, leaf, petiole and root explants, respectively. MS media supplemented with BAP (2.0 mgL^-1), NAA (1.0 mgL^-1) and GA3 (0.25 mgL^-1) best suited for multiple shoot buds initiation (82%), while maximum shoot length was formed on media with BAP (1.5 mgL^-1), NAA (0.25 mgL^-1) and Kinetin (0.5 mgL^-1). Full strength media with IAA (0.5 mgL^-1) along with IBA (0.5 mgL^-1) resulted in early roots initiation. Similarly,

SUBMITTER: Khan A 

PROVIDER: S-EPMC8654858 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature