Activity-based, bioorthogonal imaging of phospholipase D reveals spatiotemporal dynamics of GPCR-Gq signaling.

ABSTRACT: Canonically, G-protein-coupled receptor (GPCR) signaling is transient and confined to the plasma membrane (PM). Deviating from this paradigm, the parathyroid hormone receptor (PTHR1) stimulates sustained G_s signaling at endosomes. In addition to G_s, PTHR1 activates G_q signaling; yet, in contrast to the PTHR1-G_s pathway, the spatiotemporal dynamics of the G_q branch of PTHR1 signaling and its relationship to G_s signaling remain largely ill defined. Recognizing that a downstream consequence of G_q signaling is the activation of phospholipase D (PLD) enzymes, we leverage activity-based, bioorthogonal imaging tools for PLD signaling to visualize and quantify the G_q branch of PTHR1 signaling. We establish that

SUBMITTER: Liang D 

PROVIDER: S-EPMC8655016 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature