Unknown

Dataset Information

0

Tracking neuronal motility in live murine retinal explants.


ABSTRACT: The developing retina undergoes dynamic organizational changes involving significant intra-retinal motility of the encompassing cells. Here, we present a protocol for tracking retinal cell motility in live explanted mouse retinae. Although originally applied to rod and cone photoreceptors, this strategy is applicable to any fluorescently labeled cell in mouse retinae and other similar experimental retinal models. Careful tissue handling is critical for the successful acquisition of high-quality live imaging data. Further instructions for semi-automated in silico data handling are provided. For complete details on the use and execution of this protocol, please refer to Aghaizu et al. (2021).

SUBMITTER: Aghaizu ND 

PROVIDER: S-EPMC8666713 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Tracking neuronal motility in live murine retinal explants.

Aghaizu Nozie D ND   Warre-Cornish Katherine M KM   Robinson Martha R MR   Ali Robin R RR   Pearson Rachael A RA  

STAR protocols 20211209 4


The developing retina undergoes dynamic organizational changes involving significant intra-retinal motility of the encompassing cells. Here, we present a protocol for tracking retinal cell motility in live explanted mouse retinae. Although originally applied to rod and cone photoreceptors, this strategy is applicable to any fluorescently labeled cell in mouse retinae and other similar experimental retinal models. Careful tissue handling is critical for the successful acquisition of high-quality  ...[more]

Similar Datasets

| S-EPMC8384250 | biostudies-literature
| S-EPMC8508578 | biostudies-literature
| S-EPMC1257700 | biostudies-literature
| S-EPMC6509234 | biostudies-literature
| S-EPMC5344026 | biostudies-literature
| S-EPMC6974628 | biostudies-literature
| S-EPMC8713574 | biostudies-literature
| S-EPMC7915036 | biostudies-literature
| S-EPMC5909113 | biostudies-literature
| S-EPMC7564475 | biostudies-literature