Chimeric Helper-Dependent Adenoviruses Transduce Retinal Ganglion Cells and Muller Cells in Human Retinal Explants.

ABSTRACT: Purpose: Despite numerous recent advances in retinal gene therapy using adeno-associated viruses (AAVs) as delivery vectors, there remains a crucial need to identify viral vectors with the ability to transduce specific retinal cell types and that have a larger carrying capacity than AAV. In this study, we evaluate the retinal tropism of 2 chimeric helper-dependent adenoviruses (HDAds), helper-dependent adenovirus serotype 5 (HDAd5)/3 and HDAd5/35, both ex vivo using human retinal explants and in vivo using rats. Methods: We transduced cultured human retinal explants with HDAd5/3 and HDAd5/35 carrying an eGFP vector and evaluated tropism and transduction efficiency using immunohistochemistry. To assess in vivo transduction efficiency, subreti

SUBMITTER: Han IC 

PROVIDER: S-EPMC8713574 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature