Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq.
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ABSTRACT:
SUBMITTER: Philpott M
PROVIDER: S-EPMC8668430 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq.
Philpott Martin M Watson Jonathan J Thakurta Anjan A Brown Tom T Brown Tom T Oppermann Udo U Cribbs Adam P AP
Nature biotechnology 20210701 12
Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells. Barcodes and unique molecular identifiers are synthesized using dimeric nucleotide building blocks that allow error detection. We illustrate the use of the method for evaluating barcode assignment accuracy, differential isoform usage in myeloma cell lines, and ...[more]
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