Immobilization of GOx Enzyme on SiO₂-Coated Ni-Co Ferrite Nanocomposites as Magnetic Support and Their Antimicrobial and Photocatalytic Activities.

ABSTRACT: The present study used a sol-gel auto-combustion approach to make silica (SiO₂)-coated Ni-Co ferrite nanocomposites that would be used as a platform for enzyme immobilization. Using glutaraldehyde as a coupling agent, glucose oxidase (GOx) was covalently immobilized on this magnetic substrate. X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), high-resolution transmission electron microscopy (HRTEM), and fourier transform infrared spectroscopy (FTIR) was used to determine the structural analysis and morphology of Ni-Co ferrite/SiO₂ nanocomposites. FTIR spectra confirmed the binding of GOx to Ni-Co ferrite/SiO₂ nanocomposites, with a loading efficiency of around 85%. At alkaline pH and higher temperature,

SUBMITTER: Nasir Z 

PROVIDER: S-EPMC8675013 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature