Sodium hydrogen exchanger (NHE1) palmitoylation and potential functional regulation.

ABSTRACT:

Aims

Determine the effect of palmitoylation on the sodium hydrogen exchanger isoform 1 (NHE1), a member of the SLC9 family.

Main methods

NHE1 expressed in native rat tissues or in heterologous cells was assessed for palmitoylation by acyl-biotinyl exchange (ABE) and metabolic labeling with [³H]palmitate. Cellular palmitoylation was inhibited using 2-bromopalmitate (2BP) followed by determination of NHE1 palmitoylation status, intracellular pH, stress fiber formation, and cell migration. In addition, NHE1 was activated with LPA treatment followed by determination of NHE1 palmitoylation status and LPA-induced change in intracellular pH was determined in the presence and absence of preincubation with 2BP.

Key findings

In this study we demonstrate for the first

SUBMITTER: Hovde MJ 

PROVIDER: S-EPMC8692447 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature