Mycoplasma suis Alpha-Enolase Subunit Vaccine Induces an Immune Response in Experimental Animals.

ABSTRACT: Recombinant protein technology has emerged as an excellent option for vaccine development. However, prior to our study, the immune induction ability of recombinant Mycoplasma suis alpha-enolase (rMseno) in animals remained unclear. The purpose of this study was to develop a rMseno protein subunit vaccine and to determine its ability to elicit an immunological response. To accomplish this, we cloned the gene into pET-15b, expressed it in BL21 cells, and purified it. Following the establishment of immunity, the immunogenicity and potential for protection of rMseno were evaluated in mice and piglets. The results demonstrate that anti-M. suis serum recognized the pure rMseno protein in both mice and piglets as evidenced by high levels of specific anti-rMseno antibodies, significantly increased

SUBMITTER: Xue S 

PROVIDER: S-EPMC8708218 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature