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Mapping atypical UV photoproducts in vitro and across the S. cerevisiae genome.

ABSTRACT: Exposure to ultraviolet (UV) light induces DNA damage, predominantly cyclobutane pyrimidine dimers (CPD) and 6,4-photoproducts (6,4-PP), as well as rare, atypical photoproducts at thymidine-adenine (TA) sequences. We have recently shown 'TA' photoproducts are induced in UV-irradiated oligonucleotides and across the budding yeast genome. Here, we describe a protocol for mapping atypical 'TA' photoproducts in vitro and in vivo. This protocol overcomes the technical challenges involved in accurately mapping such rare photoproducts by using ultraviolet damage endonuclease (UVDE) enzymes. For complete details on the use and execution of this protocol, please refer to Laughery et al. (2020).

SUBMITTER: Bohm KA

PROVIDER: S-EPMC8715331 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Mapping atypical UV photoproducts in vitro and across the S. cerevisiae genome.

Bohm Kaitlynne A KA Sivapragasam Smitha S Wyrick John J JJ

STAR protocols 20211222 1

Exposure to ultraviolet (UV) light induces DNA damage, predominantly cyclobutane pyrimidine dimers (CPD) and 6,4-photoproducts (6,4-PP), as well as rare, atypical photoproducts at thymidine-adenine (TA) sequences. We have recently shown 'TA' photoproducts are induced in UV-irradiated oligonucleotides and across the budding yeast genome. Here, we describe a protocol for mapping atypical 'TA' photoproducts in vitro and in vivo. This protocol overcomes the technical challenges involve ...[more]

PMID: 35005641

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