In vivo metabolic effects after acute activation of skeletal muscle G_s signaling.

ABSTRACT:

Objective

The goal of this study was to determine the glucometabolic effects of acute activation of G_s signaling in skeletal muscle (SKM) in vivo and its contribution to whole-body glucose homeostasis.

Methods

To address this question, we studied mice that express a G_s-coupled designer G protein-coupled receptor (Gs-DREADD or GsD) selectively in skeletal muscle. We also identified two G_s-coupled GPCRs that are endogenously expressed by SKM at relatively high levels (β₂-adrenergic receptor and CRF₂ receptor) and studied the acute metabolic effects of activating these receptors in vivo by highly selective agonists (clenbuterol and urocortin 2 (UCN2), respectively).

Results

Acute stimulation of GsD signaling in SKM i

SUBMITTER: Meister J 

PROVIDER: S-EPMC8728399 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature