Efficient In Vitro Generation of IL-22-Secreting ILC3 From CD34⁺ Hematopoietic Progenitors in a Human Mesenchymal Stem Cell Niche.

ABSTRACT: Innate lymphoid cells (ILCs) and in particular ILC3s have been described to be vital for mucosal barrier functions and homeostasis within the gastrointestinal (GI) tract. Importantly, IL-22-secreting ILC3 have been implicated in the control of inflammatory bowel disease (IBD) and were shown to reduce the incidence of graft-versus-host disease (GvHD) as well as the risk of transplant rejection. Unfortunately, IL-22-secreting ILC3 are primarily located in mucosal tissues and are not found within the circulation, making access to them in humans challenging. On this account, there is a growing desire for clinically applicable protocols for in vitro generation of effector ILC3. Here, we present an approach for faithful generation of functionally competent human ILC3s from cord blood-deri

SUBMITTER: Bennstein SB 

PROVIDER: S-EPMC8739490 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature