Meta-analysis of human and mouse ALS astrocytes reveals multi-omic signatures of inflammatory reactive states.

ABSTRACT: Astrocytes contribute to motor neuron death in amyotrophic lateral sclerosis (ALS), but whether they adopt deleterious features consistent with inflammatory reactive states remains incompletely resolved. To identify inflammatory reactive features in ALS human induced pluripotent stem cell (hiPSC)-derived astrocytes, we examined transcriptomics, proteomics, and glutamate uptake in VCP-mutant astrocytes. We complemented this by examining other ALS mutations and models using a systematic meta-analysis of all publicly-available ALS astrocyte sequencing data, which included hiPSC-derived astrocytes carrying SOD1, C9orf72, and FUS gene mutations as well as mouse ALS astrocyte models with SOD1^G93A mutation, Tardbp deletion, and Tmem259 (

SUBMITTER: Ziff OJ 

PROVIDER: S-EPMC8744676 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature