Interlaboratory comparison of 25-hydroxyvitamin D assays: Vitamin D Standardization Program (VDSP) Intercomparison Study 2 - Part 2 ligand binding assays - impact of 25-hydroxyvitamin D₂ and 24R,25-dihydroxyvitamin D₃ on assay performance.

ABSTRACT: An interlaboratory comparison study was conducted by the Vitamin D Standardization Program (VDSP) to assess the performance of ligand binding assays (Part 2) for the determination of serum total 25-hydroxyvitamin D [25(OH)D]. Fifty single-donor samples were assigned target values for concentrations of 25-hydroxyvitamin D₂ [25(OH)D₂], 25-hydroxyvitamin D₃ [25(OH)D₃], 3-epi-25-hydroxyvitamin D₃ [3-epi-25(OH)D₃], and 24R,25-dihydroxyvitamin D₃ [24R,25(OH)₂D₃] using isotope dilution liquid chromatography-tandem mass spectrometry (ID LC-MS/MS). VDSP Intercomparison Study 2 Part 2 includes results from 17 laboratories using 32 ligand binding assays. Assay performance was evaluated using mean % bias

SUBMITTER: Wise SA 

PROVIDER: S-EPMC8748290 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature