Dcx expression defines a subpopulation of Gdf5 ⁺ cells with chondrogenic potentials in E12.5 mouse embryonic limbs.

ABSTRACT: Growth differentiation factor 5 (Gdf5) and doublecortin (Dcx) genes are both expressed in joint interzone cells during synovial joint development. In this study, we re-analyzed the single cell RNA-sequencing data (Gene Expression Omnibus GSE151985) generated from Gdf5 ⁺ cells of mouse knee joints at embryonic stages of E12.5, E13.5, E14.5, and E15.5, with a new focus on Dcx. We found that Dcx expression was enriched in clusters of Gdf5 ⁺ cells, with high expression levels of pro-chondrogenic genes including sex determining region Y-box transcription factor 5 (Sox5), Sox6, Sox9, Gdf5, versican, matrilin 4, collagen type II α 1 chain (Col2a1), Col9a1, Col9a2, and Col9a3 at

SUBMITTER: Lan R 

PROVIDER: S-EPMC8749014 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature