CRISPR/Cas9 genome editing system confirms centriolin's role in cytokinesis.

ABSTRACT:

Objective

In addition to its function as the microtubule organizing center of the cell, the centrosome has functions in many other cellular processes including primary cilia formation, DNA damage checkpoints, and cell cycle progression. But the role of individual components of the centrosome in these processes remains unclear. Previous studies used siRNA (small interfering RNA) to "knock down" protein levels of the centrosome component centriolin, resulting in failed cytokinesis. Since this approach was transient, only targeting centriolin at the mRNA level, we sought to confirm these findings by permanently disrupting the gene encoding centriolin using the CRISPR/Cas9 system of genome editing.

Results

This study provides evidence that the CRISPR/Cas9 system is capable of ef

SUBMITTER: Seronick E 

PROVIDER: S-EPMC8751070 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature