MuWU: Mutant-seq library analysis and annotation.

ABSTRACT:

Motivation

Insertional mutagenesis allows for the creation of loss-of-function mutations on a genome-wide scale. In theory, every gene can be 'knocked out' via the insertion of an additional DNA sequence. Resources of sequence-indexed mutants of plant and animal model organisms are instrumental for functional genomics studies. Such repositories significantly speed up the acquisition of interesting genotypes and allow for the validation of hypotheses regarding phenotypic consequences in reverse genetics. To create such resources, comprehensive sequencing of flanking sequence tags using protocols such as Mutant-seq requires various downstream computational tasks, and these need to be performed in an efficient and reproducible manner.

Results

Here, we present MuWU, an automated

SUBMITTER: Stocker T 

PROVIDER: S-EPMC8756183 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature