Nanofiber curvature with Rho GTPase activity increases mouse embryonic fibroblast random migration velocity.

ABSTRACT: Mechanotransduction arises from information encoded in the shape of materials such as curvature. It induces activation of small GTPase signaling affecting cell phenotypes including differentiation. We carried out a set of preliminary experiments to test the hypothesis that curvature (1/radius) would also affect cell motility due to signal pathway crosstalk. High molecular weight poly (methyl methacrylate) straight nanofibers were electrospun with curvature ranging from 41 to 1 μm-1 and collected on a passivated glass substrate. The fiber curvature increased mouse mesenchymal stem cell aspect ratio (P < 0.02) and decreased cell area (P < 0.01). Despite little effect on some motility patterns such as polarity and persistence, we found selected fiber curvatures can increase normalized random

SUBMITTER: Bowers DT 

PROVIDER: S-EPMC8759537 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature