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Multichannel dual protein sensing using amphiphilic supramolecular assemblies.

ABSTRACT: Protein sensing strategies have implications in detection of many human pathologies. Here, a supramolecular strategy for sensing two different proteins using a multichannel readout approach is outlined. Protein-ligand binding or enzymatic cleavage can both be programmed to induce supramolecular disassembly, which leads to fluorescence enhancement via aggregation-induced emission (AIE), protein-induced fluorescence enhancement (PIFE), or disassembly-induced fluorescence enhancement (DIFE). The accompanying signal change from two different fluorophores and their patterns are then used for specific protein sensing.

SUBMITTER: Gao J

PROVIDER: S-EPMC8771897 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Multichannel dual protein sensing using amphiphilic supramolecular assemblies.

Gao Jingjing J Prachyathipsakul Theeraphop T Thayumanavan S S

Chemical communications (Cambridge, England) 20211130 95

Protein sensing strategies have implications in detection of many human pathologies. Here, a supramolecular strategy for sensing two different proteins using a multichannel readout approach is outlined. Protein-ligand binding or enzymatic cleavage can both be programmed to induce supramolecular disassembly, which leads to fluorescence enhancement <i>via</i> aggregation-induced emission (AIE), protein-induced fluorescence enhancement (PIFE), or disassembly-induced fluorescence enhancement (DIFE). ...[more]

PMID: 34787137

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