Human induced pluripotent stem cells display a similar mutation burden as embryonic pluripotent cells in vivo.

ABSTRACT: Induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine, but genetic instability is a major concern. Embryonic pluripotent cells also accumulate mutations during early development, but how this relates to the mutation burden in iPSCs remains unknown. Here, we directly compared the mutation burden of cultured iPSCs with their isogenic embryonic cells during human embryogenesis. We generated developmental lineage trees of human fetuses by phylogenetic inference from somatic mutations in the genomes of multiple stem cells, which were derived from different germ layers. Using this approach, we characterized the mutations acquired pre-gastrulation and found a rate of 1.65 mutations per cell division. When cultured in hypoxic conditions, iPSCs generated from fetal ste

SUBMITTER: Hasaart KAL 

PROVIDER: S-EPMC8792070 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature