Mass spectrometry and proteome analysis to identify SARS-CoV-2 protein from COVID-19 patient swab samples.
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ABSTRACT:
SUBMITTER: Banerjee A
PROVIDER: S-EPMC8808698 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
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Mass spectrometry and proteome analysis to identify SARS-CoV-2 protein from COVID-19 patient swab samples.
Banerjee Arghya A Pai Medha Gayathri J MGJ Singh Avinash A Nissa Mehar Un MU Srivastava Sanjeeva S
STAR protocols 20220202 1
With new emerging SARS-CoV-2 strains and their increased pathogenicity, diagnosis has become more challenging. Molecular diagnosis often involves the use of nasopharyngeal swabs and subsequent real-time PCR-based tests. Although this test is the gold standard, it has several limitations; therefore, more complementary assays are required. This protocol describes how to identify SARS-CoV-2 protein from patients' nasopharyngeal swab samples. We first introduce the approach of label-free quantitativ ...[more]
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