Immunoglobulin A-specific deficiency induces spontaneous inflammation specifically in the ileum.

ABSTRACT:

Objective

Although immunoglobulin A (IgA) is abundantly expressed in the gut and known to be an important component of mucosal barriers against luminal pathogens, its precise function remains unclear. Therefore, we tried to elucidate the effect of IgA on gut homeostasis maintenance and its mechanism.

Design

We generated various IgA mutant mouse lines using the CRISPR/Cas9 genome editing system. Then, we evaluated the effect on the small intestinal homeostasis, pathology, intestinal microbiota, cytokine production, and immune cell activation using intravital imaging.

Results

We obtained two lines, with one that contained a <50 base pair deletion in the cytoplasmic region of the IgA allele (IgA tail-mutant; IgA^tm/tm) and the other that lacked the most constan

SUBMITTER: Nagaishi T 

PROVIDER: S-EPMC8809603 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature