Evaluation of Immunogenicity by Pseudovirus Neutralization Assays for SARS-CoV-2 Variants after Primary and Booster Immunization.

ABSTRACT:

Objectives

To determine the status of immune responses after primary and booster immunization for SARS-CoV-2 variants and evaluate the differences in disease resistance based upon titers of neutralizing antibodies (NAbs) against the variants.

Methods

Participants aged 18-59 years received 2 doses of inactivated COVID-19 vaccine, 14 days apart, and a booster dose after 12 months. Blood samples were collected before vaccination (baseline), 1 and 6 months after primary immunization, and at multiple instances within 21 days of the booster dose. NAbs against the spike protein of Wuhan-Hu-1 and 3 variants were measured using pseudovirus neutralization assays.

Results

Of 400 enrolled participants, 387 completed visits scheduled within 6 months of the second dose and 346 part

SUBMITTER: Xie H 

PROVIDER: S-EPMC8809669 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature