Increased plasmablasts enhance T cell-mediated beta cell destruction and promote the development of type 1 diabetes.

ABSTRACT:

Background

Although type 1 diabetes (T1D) is typically described as a T cell-mediated autoimmune disease, increasing evidence for a role of B cells has emerged. However, the pivotal disease-relevant B cell subset and its contribution to islet autoimmunity remain elusive.

Methods

The frequencies and phenotypic characteristics of circulating B cell subsets were analyzed using flow cytometry in individuals with new-onset T1D, long-term T1D, type 2 diabetes, and nondiabetic controls, and also in a prospective cohort of patients receiving mesenchymal stromal cell (MSC) transplantation. NOD mice and adoptive transfer assay were used to dissect the role of the certain B cell subset in disease progression. An in-vitro coculture system of islets with immune cells was established to e

SUBMITTER: Ling Q 

PROVIDER: S-EPMC8818172 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature