A Green Route for High-Yield Production of Tetramethylpyrazine From Non-Food Raw Materials.

ABSTRACT: 2,3,5,6-Tetramethylpyrazine (TMP) is an active pharmaceutical ingredient originally isolated from Ligusticum wallichii for curing cardiovascular and cerebrovascular diseases and is widely used as a popular flavoring additive in the food industry. Hence, there is a great interest in developing new strategies to produce this high-value compound in an ecological and economical way. Herein, a cost-competitive combinational approach was proposed to accomplish green and high-efficiency production of TMP. First, microbial cell factories were constructed to produce acetoin (3-hydroxy-2-butanone, AC), an endogenous precursor of TMP, by introducing a biosynthesis pathway coupled with an intracellular NAD⁺ regeneration system to the wild-type Escherichia coli. To further impr

SUBMITTER: Li J 

PROVIDER: S-EPMC8823705 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature