The Barley Chloroplast Mutator (cpm) Mutant: All Roads Lead to the Msh1 Gene.

ABSTRACT: The barley chloroplast mutator (cpm) is a nuclear gene mutant that induces a wide spectrum of cytoplasmically inherited chlorophyll deficiencies. Plastome instability of cpm seedlings was determined by identification of a particular landscape of polymorphisms that suggests failures in a plastome mismatch repair (MMR) protein. In Arabidopsis, MSH genes encode proteins that are in charge of mismatch repair and have anti-recombination activity. In this work, barley homologs of these genes were identified, and their sequences were analyzed in control and cpm mutant seedlings. A substitution, leading to a premature stop codon and a truncated MSH1 protein, was identified in the Msh1 gene of cpm plants. The relationship between this mutation and the presence of chlorophyll deficiencies was establ

SUBMITTER: Lencina F 

PROVIDER: S-EPMC8836938 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature