Dataset Information

Extrusion-Based Bioprinted Boron Nitride Nanotubes Reinforced Alginate Scaffolds: Mechanical, Printability and Cell Viability Evaluation.

ABSTRACT: Alginate (Alg) hydrogels are commonly used as bioinks in 3D bioprinting. However, one of the significant drawbacks of using Alg hydrogels is their unstable mechanical properties. In this study, a novel hydrogel-based ink composed of Alg reinforced with functionalised boron nitride nanotubes (f-BNNTs) was developed and systematic quantitative characterisation was conducted to validate its printability, physiochemical properties and biocompatibility. The printability, contact angle and mechanical test results indicated good structural stability of the scaffolds. The thermal stability of the scaffolds increased with the incorporation of f-BNNTs into Alg. Human embryonic kidney cells (HEK 293T) were seeded on the scaffolds and the cell viability was recorded for 24, 48 and 72 h. Quantitative studies showed a slight effect on toxicity with a higher concentration of BNNTs in scaffolds. The results suggest that the 3D printable f-BNNTs reinforced Alg could be used as bioink for tissue engineering applications with further studies on biocompatibility.

SUBMITTER: Kakarla AB

PROVIDER: S-EPMC8839966 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Extrusion-Based Bioprinted Boron Nitride Nanotubes Reinforced Alginate Scaffolds: Mechanical, Printability and Cell Viability Evaluation.

Kakarla Akesh Babu AB Kong Ing I Kong Cin C Irving Helen H

Polymers 20220126 3

Alginate (Alg) hydrogels are commonly used as bioinks in 3D bioprinting. However, one of the significant drawbacks of using Alg hydrogels is their unstable mechanical properties. In this study, a novel hydrogel-based ink composed of Alg reinforced with functionalised boron nitride nanotubes (f-BNNTs) was developed and systematic quantitative characterisation was conducted to validate its printability, physiochemical properties and biocompatibility. The printability, contact angle and mechanical ...[more]

PMID: 35160475

Similar Datasets

Project description:Background3D bioprinting cardiac patches for epicardial transplantation are a promising approach for myocardial regeneration. Challenges remain such as quantifying printability, determining the ideal moment to transplant, and promoting vascularisation within bioprinted patches. We aimed to evaluate 3D bioprinted cardiac patches for printability, durability in culture, cell viability, and endothelial cell structural self-organisation into networks.MethodsWe evaluated 3D-bioprinted double-layer patches using alginate/gelatine (AlgGel) hydrogels and three extrusion bioprinters (REGEMAT3D, INVIVO, BIO X). Bioink contained either neonatal mouse cardiac cell spheroids or free (not-in-spheroid) human coronary artery endothelial cells with fibroblasts, mixed with AlgGel. To test the effects on durability, some patches were bioprinted as a single layer only, cultured under minimal movement conditions or had added fibroblast-derived extracellular matrix hydrogel (AlloECM). Controls included acellular AlgGel and gelatin methacryloyl (GELMA) patches.ResultsPrintability was similar across bioprinters. For AlgGel compared to GELMA: resolutions were similar (200-700 μm line diameters), printing accuracy was 45 and 25%, respectively (AlgGel was 1.7x more accurate; p < 0.05), and shape fidelity was 92% (AlgGel) and 96% (GELMA); p = 0.36. For durability, AlgGel patch median survival in culture was 14 days (IQR:10-27) overall which was not significantly affected by bioprinting system or cellular content in patches. We identified three factors which reduced durability in culture: (1) bioprinting one layer depth patches (instead of two layers); (2) movement disturbance to patches in media; and (3) the addition of AlloECM to AlgGel. Cells were viable after bioprinting followed by 28 days in culture, and all BIO X-bioprinted mouse cardiac cell spheroid patches presented contractile activity starting between day 7 and 13 after bioprinting. At day 28, endothelial cells in hydrogel displayed organisation into endothelial network-like structures.ConclusionAlgGel-based 3D bioprinted heart patches permit cardiomyocyte contractility and endothelial cell structural self-organisation. After bioprinting, a period of 2 weeks maturation in culture prior to transplantation may be optimal, allowing for a degree of tissue maturation but before many patches start to lose integrity. We quantify AlgGel printability and present novel factors which reduce AlgGel patch durability (layer number, movement, and the addition of AlloECM) and factors which had minimal effect on durability (bioprinting system and cellular patch content).

Dataset Information

Extrusion-Based Bioprinted Boron Nitride Nanotubes Reinforced Alginate Scaffolds: Mechanical, Printability and Cell Viability Evaluation.

Publications

Extrusion-Based Bioprinted Boron Nitride Nanotubes Reinforced Alginate Scaffolds: Mechanical, Printability and Cell Viability Evaluation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets