Functional Nanoparticles with Magnetic 3D Covalent Organic Framework for the Specific Recognition and Separation of Bovine Serum Albumin.

ABSTRACT: Glutathione functionalized magnetic 3D covalent organic frameworks combined with molecularly imprinted polymer (magnetic 3D COF-GSH MIPs) were developed for the selective recognition and separation of bovine serum albumin (BSA). Ultrasonication was used to prepare magnetic 3D COFs with high porosity (~1 nm) and a large surface area (373 m2 g-1). The magnetic 3D COF-GSH MIP nanoparticles had an imprinting factor of 4.79, absorption capacity of 429 mg g-1, magnetic susceptibility of 32 emu g-1, and five adsorption-desorption cycles of stability. The proposed method has the advantages of a shorter equilibrium absorption time (1.5 h), higher magnetic susceptibility (32 emu g-1), and larger imprinting factor (4.79) compared with those reported from other studies. The magnetic 3D COF-GSH MIPs used with BSA had selectivity factors of 3.68, 2.76, and 3.30 for lysozyme, ovalbumin, and cytochrome C, respectively. The successful recognition and separation of BSA in a real sample analysis verified the capability of the magnetic 3D COF-GSH MIP nanoparticles.

SUBMITTER: Bettada L 

PROVIDER: S-EPMC8840370 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature