Purification and Optimization of Extracellular Lipase from a Novel Strain Kocuria flava Y4.

ABSTRACT: The exogenous lipolytic activities of Kocuria sp. have been recognized earlier but the genus further contains many more unexplored strains. In this study, the extracellular lipase activity of Kocuria flava Y4 (GenBank accession no. MT773277), isolated from Dioscorea villosa during our previous study, was regulated by different physicochemical parameters, such as pH, temperature, shaking speed, and incubation time. For efficient immobilization of the extracellular lipase, 4% sodium alginate, 50 mL of 25 nM CaCl₂.2H₂O solution, and 15 min. Hardening time of gel beads in calcium chloride was used. For the first time, K. flava Y4 lipase was purified using ammonium sulphate precipitation followed by dialysis and DEAE-Sepharose anion excha

SUBMITTER: Barik A 

PROVIDER: S-EPMC8840939 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature