Label-free visual proteomics: Coupling MS- and EM-based approaches in structural biology.

ABSTRACT: Combining diverse experimental structural and interactomic methods allows for the construction of comprehensible molecular encyclopedias of biological systems. Typically, this involves merging several independent approaches that provide complementary structural and functional information from multiple perspectives and at different resolution ranges. A particularly potent combination lies in coupling structural information from cryoelectron microscopy or tomography (cryo-EM or cryo-ET) with interactomic and structural information from mass spectrometry (MS)-based structural proteomics. Cryo-EM/ET allows for sub-nanometer visualization of biological specimens in purified and near-native states, while MS provides bioanalytical information for proteins and protein complexes without introducing

SUBMITTER: Klykov O 

PROVIDER: S-EPMC8842845 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature