Dataset Information

MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus 'Radiant' to Fade From Red to Green.

ABSTRACT: The "Spring-red-leaf" crabapple cultivar has young red leaves and mature green leaves. However, the mechanism of anthocyanin biosynthesis in crabapple leaves in spring remains unknown. In this study, Illumina RNA sequencing (RNA-Seq) was performed on Malus 'Radiant' leaf tissues in different stages of development. Twenty-two genes in the anthocyanin biosynthesis pathway and 44 MYB transcription factors (TFs) were significantly enriched among differentially expressed genes (DEGs). Three R2R3-MYB TFs in subgroup 22 of the MYB TF family, MrMYB44-like1, MrMYB44-like2, and MrMYB44-like3, were highly expressed in green leaves according to RNA-Seq and quantitative real-time quantitative PCR results. Their expression levels were negatively correlated with anthocyanin content. In transient assays, overexpression of MrMYB44-like1, MrMYB44-like2, or MrMYB44-like3 inhibited anthocyanin accumulation and reduced pigment in leaf disks of M. 'Radiant' and fruit peels of M. domestica 'Fuji.' When the conserved region of the three MrMYB44-likes was silenced, the anthocyanin biosynthesis pathway was activated and pigments increased in both tissues. Moreover, bimolecular fluorescence complementation assays showed MrMYB44-likes interacted with MrWRKY6 to form protein complexes that regulated anthocyanin biosynthesis.

SUBMITTER: Meng JX

PROVIDER: S-EPMC8843855 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of <i>Malus</i> 'Radiant' to Fade From Red to Green.

Meng Jia-Xin JX Wei Jun J Chi Ru-Fei RF Qiao Yu-Hang YH Zhou Jing J Wang Yi-Lin YL Wang Han H Li Hou-Hua HH

Frontiers in plant science 20220201

The "Spring-red-leaf" crabapple cultivar has young red leaves and mature green leaves. However, the mechanism of anthocyanin biosynthesis in crabapple leaves in spring remains unknown. In this study, Illumina RNA sequencing (RNA-Seq) was performed on <i>Malus</i> 'Radiant' leaf tissues in different stages of development. Twenty-two genes in the anthocyanin biosynthesis pathway and 44 MYB transcription factors (TFs) were significantly enriched among differentially expressed genes (DEGs). Three R2 ...[more]

PMID: 35178062

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Project description:Cinnamomum camphora, a key multifunctional tree species, primarily serves in landscaping. Leaf color is crucial for its ornamental appeal, undergoing a transformation to red that enhances the ornamental value of C. camphora. However, the molecular mechanisms underlying this transformation remain largely unexplored. In this study, green leaf (GL), color turning red leaf (RL) and whole red leaf (WRL) were obtained to measure pigment contents, while GL and RL were analyzed for transcriptomic alterations. A decline in chlorophyll content and a rise in anthocyanins were observed during the transition from green to red leaves. Using LC MS/MS, 11 types of anthocyanins showed significant accumulative differences, with cyanidin-3,5-O-diglucoside exhibiting the greatest disparity. Comparative RNA-seq identified 22,948 genes against reference genes, revealing 544 novel genes. Of these, 3,222 genes were up-regulated and 7,391 genes were down-regulated when the FPKM mean value > 1 in at least one group. The ribosome was identified as the most abundant KEGG term, with a substantial number of down-regulated differentially expressed genes (DEGs). The results indicated a downward trend in protein content, with GL exhibiting the highest protein concentration. 22, 4, and 29 DEGs were associated with chlorophyll biosynthesis, chlorophyll degradation, and anthocyanin biosynthesis, respectively. Most DEGs related to chlorophyll biosynthesis were down-regulated. SGR and SGRL, which are associated with chlorophyll degradation, exhibited opposite differential expression, resulting in a significant decrease in chlorophyll content in RL. The significantly up-regulated genes ANS and UFGT are advantageous for anthocyanin biosynthesis, contributing to the red coloration observed. Additionally, differential expression was noted in 40 R2R3-MYBs. Two MYB90 (Ccam01G003512 and Ccam01G003515) homologs of AtMYB113 were also identified showed high levels of up-regulation in RL. These findings suggest a strong correlation between pigment metabolism and transcriptome data, elucidating the mechanism that leads to the red coloration of leaves in C. camphora.

Dataset Information

MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of Malus 'Radiant' to Fade From Red to Green.

Publications

MrMYB44-Like Negatively Regulates Anthocyanin Biosynthesis and Causes Spring Leaf Color of <i>Malus</i> 'Radiant' to Fade From Red to Green.

Similar Datasets

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