Proteomic datasets of HeLa and SiHa cell lines acquired by DDA-PASEF and diaPASEF.

ABSTRACT: We present four datasets on proteomics profiling of HeLa and SiHa cell lines associated with the research described in the paper "PROTREC: A probability-based approach for recovering missing proteins based on biological networks" [1]. Proteins in each cell line were acquired by two different data acquisition methods. The first was Data Dependent Acquisition-Parallel Accumulation Serial Fragmentation (DDA-PASEF) and the second was Parallel Accumulation-Serial Fragmentation combined with data-independent acquisition (diaPASEF) [2], [3]. Protein assembly was performed following search against the Swiss-Prot Human database using Peaks Studio for DDA datasets and Spectronaut for DIA datasets. The assembled result contains identified PSMs, peptides and proteins that are above threshold for each

SUBMITTER: Huang Z 

PROVIDER: S-EPMC8844217 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature