Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration.

ABSTRACT: Glioblastoma (GBM) is the most aggressive primary brain tumor characterized by infiltrative growth of malignant glioma cells into the surrounding brain parenchyma. In this study, our analysis of GBM patient cohorts revealed a significantly higher expression of Glycosyltransferase 8 domain containing 1 (GLT8D1) compared to normal brain tissue and could be associated with impaired patient survival. Increased in vitro expression of GLT8D1 significantly enhanced migration of two different sphere-forming GBM cell lines. By in silico analysis we predicted the 3D-structure as well as the active site residues of GLT8D1. The introduction of point mutations in the predicted active site reduced its glycosyltransferase activity in vitro and consequently

SUBMITTER: Ilina EI 

PROVIDER: S-EPMC8850796 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature