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High-quality RNA improves sensitivity of SARS-CoV-2 detection by colorimetric RT-LAMP.

ABSTRACT: The global SARS-CoV-2 pandemic requires a rapid, reliable, and user-friendly diagnostic test to help control the spread of the virus. Reverse transcription and quantitative PCR (RT-qPCR) is currently the gold standard method for SARS-CoV-2 detection. Here, we develop a protocol based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and demonstrate increased sensitivity of this technique using fresh RNA extracts compared to RNA samples subjected to freezing/thawing cycles. We further compare RT-LAMP to RT-qPCR and demonstrate that the RT-LAMP approach has high sensitivity in fresh RNA extracts and can detect positive samples with Ct values between 8 and 35.

SUBMITTER: Puigmule M

PROVIDER: S-EPMC8851528 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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High-quality RNA improves sensitivity of SARS-CoV-2 detection by colorimetric RT-LAMP.

Puigmulé Marta M Coll Mònica M Pérez-Serra Alexandra A López Laura L Picó Ferran F Neto Nuria N Corona Mònica M Pinsach-Abuin Mel Lina ML Ferrer-Costa Carles C Buxó Maria M Queralt Francesc-Xavier FX Brugada Ramon R

Experimental biology and medicine (Maywood, N.J.) 20211214 3

The global SARS-CoV-2 pandemic requires a rapid, reliable, and user-friendly diagnostic test to help control the spread of the virus. Reverse transcription and quantitative PCR (RT-qPCR) is currently the gold standard method for SARS-CoV-2 detection. Here, we develop a protocol based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and demonstrate increased sensitivity of this technique using fresh RNA extracts compared to RNA samples subjected to freezing/thawing cycles ...[more]

PMID: 34903068

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