Adaptive laboratory evolution and shuffling of Escherichia coli to enhance its tolerance and production of astaxanthin.

ABSTRACT:

Background

Astaxanthin is one of the strongest antioxidants in nature and has been widely used in aquaculture, food, cosmetic and pharmaceutical industries. Numerous stresses caused in the process of a large scale-culture, such as high acetate concentration, high osmolarity, high level of reactive oxygen species, high glucose concentration and acid environment, etc., limit cell growth to reach the real high cell density, thereby affecting astaxanthin production.

Results

We developed an adaptive laboratory evolution (ALE) strategy to enhance the production of chemicals by improving strain tolerance against industrial fermentation conditions. This ALE strategy resulted in 18.5% and 53.7% increases in cell growth and astaxanthin production in fed-batch fermentation, respectivel

SUBMITTER: Lu Q 

PROVIDER: S-EPMC8851715 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature