Dataset Information

MiR-486-5p expression is regulated by DNA methylation in osteosarcoma.

ABSTRACT:

Background

Osteosarcoma is the most common primary malignant tumour of bone occurring in children and young adolescents and is characterised by complex genetic and epigenetic changes. The miRNA miR-486-5p has been shown to be downregulated in osteosarcoma and in cancer in general.

Results

To investigate if the mir-486 locus is epigenetically regulated, we integrated DNA methylation and miR-486-5p expression data using cohorts of osteosarcoma cell lines and patient samples. A CpG island in the promoter of the ANK1 host gene of mir-486 was shown to be highly methylated in osteosarcoma cell lines as determined by methylation-specific PCR and direct bisulfite sequencing. High methylation levels were seen for osteosarcoma patient samples, xenografts and cell lines based on quantitative methylation-specific PCR. 5-Aza-2'-deoxycytidine treatment of osteosarcoma cell lines caused induction of miR-486-5p and ANK1, indicating common epigenetic regulation in osteosarcoma cell lines. When overexpressed, miR-486-5p affected cell morphology.

Conclusions

miR-486-5p represents a highly cancer relevant, epigenetically regulated miRNA in osteosarcoma, and this knowledge contributes to the understanding of osteosarcoma biology.

SUBMITTER: Namlos HM

PROVIDER: S-EPMC8851731 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Publications

miR-486-5p expression is regulated by DNA methylation in osteosarcoma.

Namløs Heidi M HM Skårn Magne M Ahmed Deeqa D Grad Iwona I Andresen Kim K Kresse Stine H SH Munthe Else E Serra Massimo M Scotlandi Katia K Llombart-Bosch Antonio A Myklebost Ola O Lind Guro E GE Meza-Zepeda Leonardo A LA

BMC genomics 20220217 1

<h4>Background</h4>Osteosarcoma is the most common primary malignant tumour of bone occurring in children and young adolescents and is characterised by complex genetic and epigenetic changes. The miRNA miR-486-5p has been shown to be downregulated in osteosarcoma and in cancer in general.<h4>Results</h4>To investigate if the mir-486 locus is epigenetically regulated, we integrated DNA methylation and miR-486-5p expression data using cohorts of osteosarcoma cell lines and patient samples. A CpG i ...[more]

PMID: 35172717

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