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Characterisation and Antibacterial Properties of Novel Biodegradable Films Based on Alginate and Roselle (Hibiscus sabdariffa L.) Extract.

ABSTRACT: Composite films were prepared with alginate and roselle extract (HE) at different concentrations (1%, 3%, and 5% w/v) via solvent casting technique and analyzed in terms of physical, mechanical, and antibacterial properties. The incorporation of HE into alginate films resulted in rough and heterogeneous surface characteristics with increasing concentrations of HE. The thickness and water vapor permeability of alginate-HE composite films were significantly higher (p < 0.05) compared to pure alginate films. Moreover, water content, solubility, swelling, tensile strength, and elongation at break value of the composite films decreased (p < 0.05) with increasing concentrations of the extract. FTIR spectra revealed shifts and intensity variations in the composite films and the formation of new peaks suggesting a possible interaction between alginate and HE. Alginate-HE films exhibited good antibacterial activity against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli and Klebsiella pneumoniae) bacteria. The antibacterial effect of the films, more pronounced against Gram-positive bacteria, increased with higher amounts of HE. The resulting films may be utilised as new biodegradable, antibacterial films in the food packaging industry to prolong shelf life and preserve food safety.

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The online version of this article (10.1007/s12649-022-01710-3) contains supplementary material, which is available to authorized users.

SUBMITTER: Aydin G

PROVIDER: S-EPMC8856933 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Characterisation and Antibacterial Properties of Novel Biodegradable Films Based on Alginate and Roselle (<i>Hibiscus sabdariffa</i> L.) Extract.

Aydin Gulsum G Zorlu Elif Busra EB

Waste and biomass valorization 20220218 6

Composite films were prepared with alginate and roselle extract (HE) at different concentrations (1%, 3%, and 5% w/v) via solvent casting technique and analyzed in terms of physical, mechanical, and antibacterial properties. The incorporation of HE into alginate films resulted in rough and heterogeneous surface characteristics with increasing concentrations of HE. The thickness and water vapor permeability of alginate-HE composite films were significantly higher (<i>p</i> < 0.05) compared to pur ...[more]

PMID: 35222746

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Project description:BackgroundMultidrug-resistant (MDR) bacteria pose a significant threat to human health worldwide by increasing the harmful impact of traditional synthetic antibiotics. Traditional medicinal plants have bioactive metabolites that can significantly modulate the growth rate, cell survival, and pathogenicity of antibiotic-resistant bacteria. Hibiscus sabdariffa L., known as Roselle or Karkade, belongs to the Malvaceae family. It is well-known for its edible aromatic red/purple calyces and is extensively utilized in the food industry and pharmacological applications. H. sabdariffa calyx bioactive phytocompounds have potent therapeutic activities such as antimicrobial, antidiabetic, antiobesity, antioxidant, anti-inflammatory, and anticancer properties.MethodsThis study utilized gas chromatography-mass spectrometry (GC-MS) analysis to determine the volatile aromatic compounds that found in the hydroethanolic extract of Hibiscus sabdariffa calyces. The purpose was to verify the antibacterial properties of Roselle calyces against selective MDR clinical bacterial isolates, including A. baumanii, E. coli, K. pneumoniae, and P. aeruginosa.ResultsThe GC-MS spectrum profile revealed the presence of twenty-seven volatile organic components, including organic fatty acid derivatives, ester compounds, sugar derivatives, and terpene components. The major GC-MS fractionations and the main active chemical compositions of the hydroethanolic extract of H. sabdariffa flowers were (E)-10-Octadecenoic acid methyl ester (59.23%), 8,11-Octadecadienoic acid, methyl ester (11.51%), Butanedioic acid, 3-hydroxy-2,2-dimethyl-, diethyl ester (6.22%), Diethyl succinate/Butanedioic acid, diethyl ester (2.35%), and Heptadecanoic acid, 16-methyl-, methyl ester/Methyl isostearate (2.31%). The hydroethanolic extract of H. sabdariffa dried calyces demonstrated potent antibacterial properties (zones diameter of inhibition growth, MIC, MBC, and MBC/MIC) against selective MDR clinical bacterial isolates, such as A. baumanii, E. coli, K. pneumoniae, and P. aeruginosa, as determined by the phytochemical screening (TAC, TFC, and TPC) and antioxidant activity (DPPH). The surface morphological characteristics of the treated A. baumanii, E. coli, K. pneumoniae, and P. aeruginosa clinical isolates have been affected in comparison to the untreated forms by the hydroethanolic extract of H. sabdariffa calyces, as determined by scanning electron microscopy (SEM). In silico predictive investigation revealed that the volatile aromatic components of the hydroethanolic extract of Roselle calyces exhibited significant scoring functions, binding affinities, and non-covalent intermolecular interactions with the MenB lyase and DNA gyrase targets of E. coli. These interactions significantly enhanced the activities of the volatile aromatic components against the bacterial pathogenicity, cell survival, growth, and differentiation of selective MDR clinical bacterial isolates.ConclusionsAccording to the in vitro and in silico findings, the hydroethanolic extract of H. sabdariffa calyces has shown potentials as an effective antioxidant and antibacterial treatment. It contains volatile aromatic compounds that can modulate selective MDR Gram-negative clinical bacterial isolates.

Project description:The emergence of bacteria that are resistant to several antibiotics has represented a serious hazard to human health globally. Bioactive metabolites from medicinal plants have a wide spectrum of therapeutic possibilities against resistant bacteria. Therefore, this study was performed to investigate the antibacterial efficacy of various extracts of three medicinal plants as Salvia officinalis L., Ziziphus spina-christi L., and Hibiscus sabdariffa L. against pathogenic Gram-negative Enterobacter cloacae (ATCC13047), Pseudomonas aeruginosa (RCMB008001), Escherichia coli (RCMB004001), and Gram-positive Staphylococcus aureus (ATCC 25923), bacteria using the agar-well diffusion method. Results revealed that, out of the three examined plant extracts, the methanol extract of H. sabdariffa L. was the most effective against all tested bacteria. The highest growth inhibition (39.6 ± 0.20 mm) was recorded against E. coli. Additionally, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the methanol extract of H. sabdariffa were detected in the case of all tested bacteria. Moreover, an antibiotic susceptibility test revealed that all tested bacteria showed multidrug resistance (MDR). While 50% of tested bacteria were sensitive and 50% were intermediately sensitive to piperacillin/tazobactam (TZP) based on the inhibition zone but still less than the extract. Synergistic assay demonstrated the promising role of using a combination of H. sabdariffa L. and (TZP) against tested bacteria. A surface investigation using a scanning electron microscope of the E. coli treated with TZP, extract, or a combination of the two revealed extremely considerable bacterial cell death. In addition, H. sabdariffa L. has a promising anticancer role versus Caco-2 cells with IC50 of 17.51 ± 0.07 µg/mL and minimal cytotoxicity upon testing versus Vero cells with CC50 of 165.24 ± 0.89 µg/mL. Flow cytometric analysis confirmed that H. sabdariffa extract significantly increased the apoptotic rate of Caco-2-treated cells compared to the untreated group. Furthermore, GC-MS analysis confirmed the existence of various bioactive components in the methanol hibiscus extract. Utilizing molecular docking with the MOE-Dock tool, binding interactions between n-Hexadecanoic acid, hexadecanoic acid-methyl ester, and oleic acid, 3-hydroxypropyl ester were evaluated against the target crystal structures of E. coli (MenB) (PDB ID:3T88) and the structure of cyclophilin of a colon cancer cell line (PDB ID: 2HQ6). The observed results provide insight into how molecular modeling methods might inhibit the tested substances, which may have applications in the treatment of E. coli and colon cancer. Thus, H. sabdariffa methanol extract is a promising candidate to be further investigated for developing alternative natural therapies for infection treatment.