Introduction and expression of PIK3CA^E545K in a papillary thyroid cancer BRAF^V600E cell line leads to a dedifferentiated aggressive phenotype.

ABSTRACT: Anaplastic thyroid cancer (ATC) is a rare, aggressive form of undifferentiated thyroid cancer, which exhibits rapid progression and is almost universally fatal. At least a subset of ATC is thought to arise from pre-existing well-differentiated thyroid cancer, most frequently papillary thyroid cancer (PTC). While PIK3CA mutations are rare in PTC, they are common in ATC and tend to co-occur with BRAF mutations. This provided the rationale for our study to identify the potential role of PIK3CA mutations in the progression from well-differentiated to undifferentiated thyroid cancer. We introduced PIK3CA^E545K into the LAM1 PTC cell line, which carries a BRAF^V600E mutation. In culture, the engineered cell line (LAM1:PIK3CA^E545K) proliferated faster and demonstrat

SUBMITTER: Pinto N 

PROVIDER: S-EPMC8862267 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature