Dataset Information

Durable T-cellular and humoral responses in SARS-CoV-2 hospitalized and community patients.

ABSTRACT:

Background

Neutralizing antibodies are important for protection against the pandemic SARS-CoV-2 virus, and long-term memory responses determine the risk of re-infection or boosting after vaccination. T-cellular responses are considered important for partial protection against novel variants of concern.

Methods

A prospective cohort of hospitalized (n = 14) and community (n = 38) patients with rt-PCR confirmed SARS-CoV-2 infection were recruited. Blood samples and clinical data were collected when diagnosed and at 6 months. Serum samples were analyzed for SARS-CoV-2-spike specific antibodies using ELISA (IgG, IgA, IgM), pseudotype neutralization and microneutralization assays. Peripheral blood mononuclear cells were investigated for virus-specific T-cell responses in the interferon-γ and interleukin-2 fluorescent-linked immunosorbent spot (FluroSpot) assay.

Results

We found durable SARS-CoV-2 spike- and internal protein specific T-cellular responses in patients with persistent antibodies at 6 months. Significantly higher IL-2 and IFN-γ secreting T-cell responses as well as SARS-CoV-2 specific IgG and neutralizing antibodies were detected in hospitalized compared to community patients. The immune response was impacted by age, gender, comorbidity and severity of illness, reflecting clinical observations.

Conclusions

SARS-CoV-2 specific T-cellular and antibody responses persisted for 6 months post confirmed infection. In previously infected patients, re-exposure or vaccination will boost long-term immunity, possibly providing protection against re-infection with variant viruses.

SUBMITTER: Mohn KG

PROVIDER: S-EPMC8863217 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

Durable T-cellular and humoral responses in SARS-CoV-2 hospitalized and community patients.

Mohn Kristin G-I KG Bredholt Geir G Zhou Fan F Madsen Anders A Onyango Therese B TB Fjelltveit Elisabeth B EB Jalloh Sarah L SL Brokstad Karl A KA Cantoni Diego D Mayora-Neto Martin M Temperton Nigel N Langeland Nina N Cox Rebecca J RJ

PloS one 20220222 2

<h4>Background</h4>Neutralizing antibodies are important for protection against the pandemic SARS-CoV-2 virus, and long-term memory responses determine the risk of re-infection or boosting after vaccination. T-cellular responses are considered important for partial protection against novel variants of concern.<h4>Methods</h4>A prospective cohort of hospitalized (n = 14) and community (n = 38) patients with rt-PCR confirmed SARS-CoV-2 infection were recruited. Blood samples and clinical data were ...[more]

PMID: 35192617

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Dataset Information

Durable T-cellular and humoral responses in SARS-CoV-2 hospitalized and community patients.

Background

Methods

Results

Conclusions

Publications

Durable T-cellular and humoral responses in SARS-CoV-2 hospitalized and community patients.

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