Dataset Information

Integrating network pharmacology and experimental validation to decipher the mechanism of the Chinese herbal prescription JieZe-1 in protecting against HSV-2 infection.

ABSTRACT:

Context

The Chinese herbal prescription JieZe-1 (JZ-1) is effective against HSV-2 (Herpes simplex virus type 2) infection. However, its mechanism remains unclear.

Objective

To explore the mechanism of JZ-1 in protecting against HSV-2 infection.

Materials and methods

Using the methods of network pharmacology, the hub components and targets were screened and functionally enriched. We established a genital herpes (GH) mouse model and observe the disease characteristics. Then, the GH mice in different groups (10 per/group) were treated with 20 μL JZ-1 gel (2.5, 1.5, and 0.5 g/mL), acyclovir gel (0.03 g/mL), or plain carbomer gel twice a day. The symptom score, vulvar histomorphology, and virus load were measured. The critical proteins of caspase-1-dependent pyroptosis were analysed by microscopy, co-immunoprecipitation, western blotting, and ELISA. Molecular docking was also performed.

Results

Network pharmacology analysis identified 388 JZ-1 targets related to HSV-2 infection, with 36 hub targets and 21 hub components screened. The TCID₅₀ of HSV-2 was 1 × 10^-7/0.1 mL. JZ-1 gel (2.5 g/mL) can effectively reduce the symptom score (81.23%), viral load (98.42%) and histopathological changes, and significantly inhibit the proteins expression of caspase-1-dependent pyroptosis in GH mice (p< 0.05). The molecular docking test showed a good binding potency between 11 components and caspase-1 or interleukin (IL)-1β.

Discussion and conclusions

The present study demonstrated that JZ-1 protected mice from HSV-2 infection and inhibit the caspase-1-dependent pyroptosis in GH mice. It is of significance for the second development of JZ-1 and the exploration of new drugs.

SUBMITTER: Liu T

PROVIDER: S-EPMC8865133 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Publications

Integrating network pharmacology and experimental validation to decipher the mechanism of the Chinese herbal prescription JieZe-1 in protecting against HSV-2 infection.

Liu Tong T Shao Qingqing Q Wang Wenjia W Ma Yonggui Y Liu Tianli T Jin Ximing X Fang Jianguo J Huang Guangying G Chen Zhuo Z

Pharmaceutical biology 20221201 1

<h4>Context</h4>The Chinese herbal prescription JieZe-1 (JZ-1) is effective against HSV-2 (<i>Herpes simplex</i> virus type 2) infection. However, its mechanism remains unclear.<h4>Objective</h4>To explore the mechanism of JZ-1 in protecting against HSV-2 infection.<h4>Materials and methods</h4>Using the methods of network pharmacology, the hub components and targets were screened and functionally enriched. We established a genital herpes (GH) mouse model and observe the disease characteristics. ...[more]

PMID: 35180012

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Dataset Information

Integrating network pharmacology and experimental validation to decipher the mechanism of the Chinese herbal prescription JieZe-1 in protecting against HSV-2 infection.

Context

Objective

Materials and methods

Results

Discussion and conclusions

Publications

Integrating network pharmacology and experimental validation to decipher the mechanism of the Chinese herbal prescription JieZe-1 in protecting against HSV-2 infection.

Similar Datasets

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