ATP Synthase K⁺- and H⁺-Fluxes Drive ATP Synthesis and Enable Mitochondrial K⁺-"Uniporter" Function: I. Characterization of Ion Fluxes.

ABSTRACT: ATP synthase (F₁F_o) synthesizes daily our body's weight in ATP, whose production-rate can be transiently increased several-fold to meet changes in energy utilization. Using purified mammalian F₁F_o-reconstituted proteoliposomes and isolated mitochondria, we show F₁F_o can utilize both ΔΨ_m-driven H⁺- and K⁺-transport to synthesize ATP under physiological pH = 7.2 and K⁺ = 140 mEq/L conditions. Purely K⁺-driven ATP synthesis from single F₁F_o molecules measured by bioluminescence photon detection could be directly demonstrated along with simultaneous measurements of unitary K⁺ currents by voltage clamp, both blocked by specific F_o i

SUBMITTER: Juhaszova M 

PROVIDER: S-EPMC8867323 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature