Bufalin suppresses ovarian cancer cell proliferation via EGFR pathway.

ABSTRACT:

Background

Previous studies have shown that bufalin exerts antitumor effects through various mechanisms. This study aimed to determine the antineoplastic mechanism of bufalin, an extract of traditional Chinese medicine toad venom, in ovarian cancer.

Methods

The 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide (MTT), 5-ethynyl-2'-deoxyuridine (EdU), and colony formation assays were used to investigate the antiproliferative effect of bufalin on the ovarian cancer cell line SK-OV-3. Molecular docking was used to investigate the combination of bufalin and epidermal growth factor receptor (EGFR) protein. Western blotting was performed to detect the expression of EGFR protein and its downstream targets.

Results

Bufalin inhibited the proliferation of SK-OV-3 cel

SUBMITTER: Dou L 

PROVIDER: S-EPMC8869555 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature