ADCK2 Knockdown Affects the Migration of Melanoma Cells via MYL6.

ABSTRACT:

Background

ADCK2 is a member of the AarF domain-containing kinase family, which consists of five members, and has been shown to play a role in CoQ metabolism. However, ADCKs have also been connected to cancer cell survival, proliferation and motility. In this study, we investigated the role of ADCK2 in melanoma.

Methods

The effect of ADCK2 on melanoma cell motility was evaluated by a scratch assay and a transwell invasion assay upon siRNA-mediated knockdown or stable overexpression of ADCK2.

Results

We found that high levels of intratumoral ADCK2 and MYL6 are associated with a higher survival rate in melanoma patients. Knocking down ADCK2 resulted in enhanced cell migration of melanoma cells. Moreover, ADCK2-knockdown cells adopted a more dedifferentiated phenotype. A

SUBMITTER: Vierthaler M 

PROVIDER: S-EPMC8869929 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature