UPLC-PDA-ESI-QTOF-MS/MS fingerprint of purified flavonoid enriched fraction of Bryophyllum pinnatum; antioxidant properties, anticholinesterase activity and in silico studies.

ABSTRACT:

Context

Bryophyllum pinnatum (Lam.) Oken (Crassulaceae) is used traditionally to treat many ailments.

Objectives

This study characterizes the constituents of B. pinnatum flavonoid-rich fraction (BPFRF) and investigates their antioxidant and anticholinesterase activity using in vitro and in silico approaches.

Materials and methods

Methanol extract of B. pinnatum leaves was partitioned to yield the ethyl acetate fraction. BPFRF was isolated from the ethyl acetate fraction and purified. The constituent flavonoids were structurally characterized using UPLC-PDA-MS². Antioxidant activity (DPPH), Fe²⁺-induced lipid peroxidation (LP) and anticholinesterase activity (Ellman's method) of the BPFRF and standards (ascorbic

SUBMITTER: Ogidigo JO 

PROVIDER: S-EPMC8871626 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature